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Cold acclimation responsiveness of GPI-APs. Non-acclimated (NA) and cold-acclimated (CA) PM samples (equivalent of 2mg of protein) were incubated with or without PI-PLC. After the treatment, the protein content of each fraction was determined by Bradford assay (n=4; A). Alternatively, the proportions of identified GPI-APs in the total proteins in each sample were calculated based on the abundance of each protein obtained from the quantitative proteomic approach (n=3–7; B).
