Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 20;67(17):5217–5232. doi: 10.1093/jxb/erw288

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig. 1. — Growth arrest and premature senescence phenotypes of BOP1- and WDR12-silenced plants. (A) Growth arrest phenotype of Arabidopsis dexamethasone (DEX)-inducible BOP1 (#7 and #10) and WDR12 (#8 and #10) RNAi lines. Seedlings were germinated on MS media that contained either ethanol (–DEX) or 10 μM DEX. (B) Retarded root growth and premature senescence of aerial tissues. DEX-inducible PES (#28), BOP1 (#7), and WDR12 (#8) RNAi seedlings were grown on MS media and then transferred to (–)DEX or (+)DEX media for vertical growth. Scale bars are 9mm. (C) Root length measurements in RNAi seedlings 3, 6, and 9 d after transfer to (–)DEX or (+)DEX media. Each data point represents the mean ± SD (n=20). Asterisks denote statistical significance as follows: *, P≤0.05; **, P≤0.01. (D) Premature senescence of the inflorescence in DEX-treated RNAi plants.

(E–G) Relative transcript levels in RNAi lines. Real-time quantitative RT-PCR analyses were performed on two independent PES (E), BOP1 (F), and WDR12 (G) RNAi lines. Transcript levels were quantified relative to (–)DEX samples using UBC10 mRNA levels as a control. Each value represents the mean ±SD of three replicates per experiment. (This figure is available in colour at JXB online.)