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. 2016 Jul 20;67(17):5217–5232. doi: 10.1093/jxb/erw288

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© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Translocation of PeBoW proteins into the nucleoplasm in response to drug treatment. (A) Effects of mycophenolic acid (MPA). Nicotiana benthamiana leaves were agroinfiltrated with GFP fusion constructs and then treated with MPA (20 µM) for 0, 8, and 20h. Merged images with DAPI-stained nuclei are shown. Scale bars are 5 µm. (B) Effects of actinomycin-D (5 µM) after 0, 8, and 20h treatment. (C) Effects of methyl methanesulfonate (MMS; 0.03%) after 0, 2, and 4h treatment. (D–F) Real-time quantitative RT-PCR. Transcript levels of PES (D), BOP1 (E), and WDR12 (F) after drug treatment were compared with those prior to treatment (–). The UBC10 mRNA level was used as a control. (This figure is available in colour at JXB online.)