Table 1.
Nodulation phenotype of LjGlb1-1 mutant lines
Seedlings were inoculated with M. loti MAFF303099 (nodule number and nitrogenase activity) or its DsRed derivative (ITs) and were grown on nitrogen-free Fåhraeus medium. Nitrogenase (acetylene reduction) activity (ARA) of detached nodules was measured after 4 weeks and is expressed as nmol C2H4 produced min−1 mg−1 FW. The numbers of ITs were counted after 2 weeks and are expressed per centimeter of root.
| WT | A102V | E127K | 96642 | |
|---|---|---|---|---|
| Nodule number | 7.17±0.51 a | 5.22±0.30 b | 2.67±0.28 c | 4.93±0.42 b |
| ARA | 11.18±0.72 a | 9.64±0.51 ab | 2.71±0.60 c | 8.54±0.69 b |
| Incipient ITs | 2.88±0.13 a | 4.43±0.42 b | 8.15±1.28 b | 4.52±0.61 b |
| Long ITs | 32.08±1.41 a | 17.11±0.98 b | 5.98±0.93 c | 11.57±0.82 d |
| Total ITs | 34.95±1.42 a | 21.54±1.30 b | 14.00±2.05 c | 16.09±1.21 c |
Means (± SE, n=9 for ARA and n=14–22 for the other parameters) denoted by the same letter do not significantly differ (P=0.05) based on Duncan’s multiple range test.