Figure 4.

Glucocorticoids prevent erythroid progenitor loss and decrease differentiation in Rps19‐deficiency. (A) Healthy and Rps19‐deficient KIT‐enriched faetal liver cells cultured with and without dexamethasone for 24, 48 or 72 h and then plated for colony assay, in three separate experiments. (B) Flow cytometric analysis of the erythroid differentiation markers CD44 and TER119 in Rps19‐deficient and healthy cultures with or without Dexamethasone treatment. Shown here are representative plots for each condition and time point from three separate experiments. (C) Quantification of data from the flow cytometric experiment in (B). (D) Cell cycle distribution analysis of cells cultured for 72 h. Displayed here is mean with standard deviation and paired student's t‐test was used for statistical analysis.