Figure 5.

SAHA represses proliferation of normal intestinal HIEC cells. To assess proliferation (A), HIEC cells were treated with SAHA or DMSO alone for 48 h followed by BrdU assay. Data are represented as the percentage of BrdU positive nuclei relative to the total number of nuclei stained with DAPI. To determine apoptosis (B), caspase‐3 activity was assessed on newly confluent HIEC cells treated 4 days with DMSO alone (Control) or SAHA. In both sets of analyses, data are expressed as the mean ± SEM from three independent experiments. ****P ˂ 0.0005. N.S.: Non‐significant.