Table 2.
Sensitivity of real-time PCR assays compared with the reference method
| Analyte | Real-time PCR assay limits of detection* | ||||
|---|---|---|---|---|---|
| Reference method | Test 1 | Test 2 | Test 3 | Average | |
| BFV | 10e2 | 10e1 | 10e2 | 10e4 | 10e2 |
| MVEV | 10e6 | 10e4 | 10e6 | 10e5 | 10e5 |
| RRV | 10e1 | 10e0 | 10e0 | 10e0 | 10e0 |
| KUNV | 10e4 | 10e3 | 10e5 | 10e4 | 10e4 |
BFV = Barmah Forest virus; KUNV = Kunjin virus; MVEV = Murray Valley encephalitis virus; PCR = polymerase chain reaction; RRV = Ross River virus.
*
Using positive reference laboratory control material as the comparison and three replicates each in a 10-fold dilution series, lowest dilution of serial 10-fold dilution produce a positive PCR result. Singleplex real-time arbovirus PCR assay targets BFV, MVEV, RRV, and KUNV.