Table 3.
Real-time PCR assay performance of Australian arbovirus PCR field assays under reference laboratory conditions
| Test performance* | ||||
|---|---|---|---|---|
| BFV | KUN | MVEV | RRV | |
| Sensitivity | 1.00 | 1.00 | 0.62 | 0.50 |
| Specificity | 1.00 | 0.95 | 1.00 | 1.00 |
| PPV | 1.00 | 0.82 | 1.00 | 1.00 |
| NPV | 1.00 | 1.00 | 0.93 | 0.91 |
BFV = Barmah Forest virus; KUNV = Kunjin virus; MVEV = Murray Valley encephalitis virus; PCR = polymerase chain reaction; PPV = positive predictive value; RRV = Ross River virus; NPV = negative predictive value.
*
One sample was found to be inhibitory for the PCR assay.