. 2016 Sep 7;95(3):633–638. doi: 10.4269/ajtmh.15-0878

Table 4.

Arbovirus detection in mosquitoes trapped in May 2010 in Far North Queensland uplands using field deployable PCR methods

Laboratory no.	Date of collection	Date of testing	Description	Trap no.	Mosquito species in pool	No. of insects tested	Real-time PCR
Laboratory no.	Date of collection	Date of testing	Description	Trap no.	Mosquito species in pool	No. of insects tested	BFV^†	KUNV^†	MVEV^†	RRV^†
1	May 14	May 17	Base camp vicinity	3	Mixed culicines	5	BFV 39^*	NA^‡	NA^‡	NA^‡
2	May 15	May 17	Airstrip	5	Culex annulirostris	12	Neg	NA^‡	NA^‡	NA^‡
3	May 15	May 17	Camp area	6	Mixed	10	BFV	NA^‡	NA^‡	NA^‡
4	May 15	May 17	Camp area	6	Cx. annulirostris	5	BFV	NA^‡	NA^‡	NA^‡
5	May 15	May 17	Creek junction	7	Culex quinquefasciatus	12	BFV	NA^‡	NA^‡	NA^‡
6	May 15	May 17	Creek junction	7	Mixed culicines including Aedes	7	BFV 36	NA^‡	NA^‡	NA^‡
7	May 15	May 17	Creek junction	7	Cx. annulirostris	20	BFV 38	NA^‡	NA^‡	NA^‡
8	May 16	May 17	Homestead	9	Mixed mosquitoes	18	BFV 39	NA^‡	NA^‡	NA^‡
9	May 16	May 17	Homestead	9	Cx. annulirostris	20	BFV 39	NA^‡	NA^‡	NA^‡
10	May 15	May 17	Range control buildings	8	Mixed culicines	6	Neg	NA^‡	NA^‡	NA^‡
11	May 16	May 17	Creek	11	Mixed culicines	6	Neg	NA^‡	NA^‡	NA^‡
12	May 16	May 17	Creek	11	Cx. annulirostris	12	Neg	NA^‡	NA^‡	NA^‡
13	May 16	May 17	Creek	12	Cx. annulirostris	10	BFV	NA^‡	NA^‡	NA^‡
14	May 16	May 17	Creek	12	Mixed mosquito spp.	9	Neg	NA^‡	NA^‡	NA^‡
15	May 14	May 17	Creek base camp vicinity	2	Cx. annulirostris	10	Neg	NA^‡	NA^‡	NA^‡
16	May 14	May 17	Case camp vicinity	2	Mixed Aedes spp.	6	BFV 38	NA^‡	NA^‡	NA^‡
17	May 16	May 17	–	10	Cx. annulirostris	12	Neg	NA^‡	NA^‡	NA^‡
18	May 17	May 17	Non-template control	–	NTC	–	Neg	NA^‡	NA^‡	NA^‡
19	May 17	May 17	Control tower	13	Cx. annulirostris	5	NA^§	Neg	Neg	Neg
20	May 17	May 17	Hut	15	Cx. annulirostris	12	NA^§	Neg	Neg	Neg
21	May 17	May 17	Airfield	5	Anopheles annulipes	14	NA^§	Neg	Neg	Neg
22	May 14	May 17	Base camp vicinity	2	An. annulipes	9	NA^§	Neg	Neg	RRV
23	May 14	May 17	Woodland	4	An. annulipes	2	NA^§	Neg	Neg	Neg
24	May 16	May 17	Camp site	10	Cx. annulirostris	25	NA^§	Neg	Neg	Neg
25	May 17	May 17	Hut	15	Aedes linneatipennis	13	NA^§	Neg	Neg	Neg
26	May 17	May 17	Control tower	13	Aedes tremulus	17	NA^§	Neg	Neg	Neg
27	May 18	May 18	Buildings	16	Cx. annulirostris	4	NA^§	Neg	MVEV 37	RRV 34
28	May 18	May 18	Buildings	16	An. annulipes	7	NA^§	Neg	MVEV 26	Neg
29	May 18	May 18	Camp site	17	Cx. annulirostris	11	NA^§	Neg	MVEV 33	Neg
30	May 18	May 18	Creek	18	Cx. annulirostris	3	BFV 16	Neg	MVEV 36	RRV 37
31	May 18	May 18	Creek	18	Culex bitaeniorhynchus	10	BFV 17	Neg	MVEV 33	RRV 34
32	May 18	May 18	Creek	18	Coquillettidia xanthogaster	20	BFV 17	Neg	MVEV 34	RRV 36
33	May 18	May 18	Camp site	19	Cx. annulirostris	9	NA^§	Neg	MVEV 30	RRV 32

BFV = Barmah Forest virus; KUNV = Kunjin virus; MVEV = Murray Valley Encephalitis virus; NA = not applicable; Neg = negative; PCR = polymerase chain reaction; RRV = Ross River Virus.

Positive qPCR assay results expressed as cycling threshold (Ct) values when < 40.0, and negative when Ct ≥ 40.0.

^†

Traps per arbovirus were BFV = 16, KUNV = 10, MVEV = 10, and RRV = 10. Trap numbers correspond to numbered boxes in Figure 1.

^‡

Limited analysis of tested mosquito extracts for only BFV due to expected low sensitivity of testing small numbers of trapped insects (below preferred minimum aliquot of 25 insects).

^§

Exhaustion of BFV PCR master mix prevented analysis of all second series of mosquito pools.