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. 2016 Sep 7;12(9):e1006287. doi: 10.1371/journal.pgen.1006287

Fig 3. RsiVS169W, P259A, Y261A is unable to bind lysozyme.

Fig 3

Representative run of isothermal titration calorimetry (ITC) experiments conducted with A. 6xHis-2xFlag-RsiV59-285 and HEW lysozyme or B. 6xHis-2xFlag-RsiV59-285, S169W, P259A, Y261A and HEW lysozyme. An interaction was seen with the WT construct, but not with the triple mutant. C. HEW lysozyme only injection control. RsiV was loaded in the cell at a concentration of 0.01 mM and lysozyme was loaded in the syringe at a concentration of 0.1 mM.