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. 2016 Sep 7;12(9):e1006287. doi: 10.1371/journal.pgen.1006287

Fig 5. RsiV binding to lysozyme is required for site-1 cleavage of RsiV.

Fig 5

Site-1 cleavage of RsiV mutants in response to increasing concentration of lysozyme. Overnight B. subtilis strains were grown to OD600 of 1 in LB IPTG. Strains were divided into 1.5 ml aliquots and incubated with increasing concentrations of lysozyme (0, 0.01, 0.1, 1, and 2 μg/mL) for 10 minutes. Following lysozyme exposure, cells were pelleted, resuspended in 100 μl sample buffer, and immunoblotted with anti-RsiV and anti-σA. The blots are labelled on the right with either α-RsiV59-285 or α-σA. The FL arrow denotes full length RsiV. The C arrow denotes cleaved extracellular domain of RsiV. WT = JLH402; S169W P259A Y261A refers to JLH1312; S169W refers to JLH1271; P259A Y261A refers to JLH1343; P259A refers to JLH1481; S169W P259A refers to JLH1326; Y261A refers to JLH1342; S169W Y261A refers to JLH1504.