Table 7. Strain list.
| Strain | Genotype | Referencea |
|---|---|---|
| E.coli | ||
| BL21(DE3) | E. coli fhuA2 [lon] ompT gal (λ DE3) [dcm] ΔhsdS λDE3 (sBamHIo ΔEcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 Δnin5) | |
| B. subtilis | ||
| PY79 | Prototrophic derivative of B. subtilis 168 | [76] |
| CDE1546 | PY79 pyrD::PsigV-lacZ (cat) | [5] |
| CDE1563 | PY79 ΔsigVrsiV::kan | [11] |
| JLH402 | PY79 amyE::Phs-rsiV (spec) ΔsigVrsiV::kan | [11] |
| JLH1312 | PY79 amyE::Phs-rsiVS169W,P259A, Y261A (spec) ΔsigVrsiV::kan | |
| JLH1271 | PY79 amyE::Phs-rsiVS169W (spec) ΔsigVrsiV::kan | |
| JLH1481 | PY79 amyE::Phs-rsiVP259A (spec) ΔsigVrsiV::kan | |
| JLH1342 | PY79 amyE::Phs-rsiVY261A (spec) ΔsigVrsiV::kan | |
| JLH1343 | PY79 amyE::Phs-rsiVP259A, Y261A (spec) ΔsigVrsiV::kan | |
| JLH1326 | PY79 amyE::Phs-rsiVS169W, P259A (spec) ΔsigVrsiV::kan | |
| JLH1504 | PY79 amyE::Phs-rsiVS169W, Y261A (spec) ΔsigVrsiV::kan | |
| JLH1412 | PY79 pyrD::PsigV-lacZ (cat) rsiVS169W, P259A, Y261A | |
| JLH1413 | PY79 pyrD::PsigV-lacZ (cat) rsiVS169W | |
| JLH1380 | PY79 pyrD::PsigV-lacZ (cat) rsiVP259A | |
| JLH1510 | PY79 pyrD::PsigV-lacZ (cat) rsiVY261A | |
| JLH1372 | PY79 pyrD::PsigV-lacZ (cat) rsiV P259A, Y261A | |
| JLH1507 | PY79 pyrD::PsigV-lacZ (cat) rsiVS169W, Y261A | |
| JLH1362 | PY79 pyrD::PsigV-lacZ (cat) rsiVH137A K139A | |
| JLH1536 | PY79 pyrD::PsigV-lacZ (cat) rsiVS169W, P259A | |
| JLH1080 | PY79 amyE::Phs-rsiVT270A (spec) pyrD::PsigV-lacZ (cat) | |
| JLH1081 | PY79 amyE::Phs-rsiVT270A (spec) ΔsigVrsiV;:kan | |
| JLH1101 | PY79 amyE::Phs-rsiVS131P (spec) pyrD::PsigV-lacZ (cat) | |
| JLH1102 | PY79 amyE::Phs-rsiVS131P (spec) ΔsigVrsiV::kan |
a This study, unless otherwise noted.