(A) Only ~5% of tumor cells and microbeads disseminated to the spine. No significant difference was found between the groups. (B) Long bones revealed dissemination fractions between ~10–25%. TRAMP-C2-luc disseminated significantly higher to long bones compared to LLC1-luc cells (LLC1-luc vs. TRAMP-C2-luc: p = 0.0406). No other significant dissemination differences were found. (C) Low tumor cell and microbeads dissemination was found in the thorax (~5–10%). No significant difference was found between the groups. (D) Dissemination to the cranium was ~2–6%. No significant dissemination differences were found. Mean ± SD, (B16-luc n = 4, LLC1-luc n = 5, TRAMP-C2-luc n = 5, 7 μm beads n = 5, 16 μm beads n = 5) for all experiments shown, one way ANOVA with Bonferroni post hoc analysis.