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. Author manuscript; available in PMC: 2016 Sep 7.
Published in final edited form as: Hum Mutat. 2015 Apr 27;36(6):599–610. doi: 10.1002/humu.22775

Figure 2.

Figure 2

Homodimerization of NR2E3 wild-type and variant proteins. HEK293T cells were transiently transfected with 1 μg of expression plasmids for RLuc-NR2E3 wild-type (WT) or variant fusion proteins and with increasing amounts (0.1–1.5 μg) of expression plasmids for GFP-NR2E3 wild-type (WT) or variant fusion proteins (GFP μg). BRET2 ratios for wild-type homodimers are represented by black circles in all panels (A–P). The localization of the different variants is described in the main text. All experiments were performed four times in duplicates and SEM indicated. Two-way ANOVA (interaction) was used to determine variant BRET2 ratio profiles to be significantly different from the wild-type profile. *: p<0.05; ***: p<0.001; ****: p<0.0001.