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. 2016 Sep 7;11(9):e0162272. doi: 10.1371/journal.pone.0162272

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© 2016 Zhao et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — (A) Expression analysis of the recombinant As-G1LEA protein. Lane M: Protein markers from 10 to 120 kDa. Lanes 1–4 show the expression of the recombinant As-G1LEA protein from four induction treatments (1 mM IPTG at 37C, 1 mM IPTG at 30C, 0.25 mM IPTG at 37C, and 0.25 mM IPTG at 30C, respectively). Lane 5: Total proteins from non-induced cells. Lane 6: total proteins from induced cells harboring pET-30a (control). (B) Determination of the solubility of the As-G1LEA recombinant protein. Lane 1: Total proteins from non-induced cells. Lane 2: Total As-G1LEA recombinant protein. Lane 4: Soluble fraction of the lysate from induced cells harboring pET-30a-G1LEA. Lane 4: Insoluble fraction of the lysate from induced cells harboring pET-30a-G1LEA. (C) Lane 1: Unpurified, induced As-G1LEA recombinant protein. Lane 2: Flow-through proteins. Lane 3: 20mM imidazole eluate. Lane 4: 40mM imidazole eluate. Lane 5: 60 mM imidazole eluate. (D) Western blot showing specific binding of the antibody to the A. sinica protein.