Fig 12. Immunolocalization of As-G3LEA at the Gastrula stage, embryonic stage and sub-adult stage in Artemia sinica.

Paraffin sections of these stages were prepared for immunofluorescence microscopy, as described in the experimental procedures. A1, B1 and C1 indicate single-labeling with polyclonal anti-G3LEA; A2, B2 and C2 indicate single-labeled with DAPI (cell nuclear blue fluorescent probe); A3, B3 and C3 represent the image overlay of the samples dual-labeled with polyclonal anti-G3LEA and DAPI. A4, B4 and C4 represent the image overlay of control group samples single-labeled with the secondary antibody. The arrows indicate parts with a positive signal.