Table 2. Primer Sequences for Genes Quantified by Real-Time PCRa.
Gene | GeneBank # | Sequence |
---|---|---|
Actb | NM_007393 | Forward 5’-TACAGCTTCACCACCACAGC-3’ Reverse 3’-CTTCTCCAGGGAGGAAGAGG-5’ |
B2m | NM_009735 | Forward 5’-TGGTGCTTGTCTCACTGACC-3’ Reverse 3’-CGGGTGGAACTGTGTTACG-5’ |
Hsp90ab1 | NM_008302 | Forward 5’-ACTGCTCTGCTCTCCTCTGG-3’ Reverse 3’-GGGATCTCATCAGGAACAGC-5’ |
Cck | NM_031161 | Forward 5’-ACTGCTAGCGCGATACATCC-3’ Reverse 3’-CCCACTACGATGGGTATTCG-5’ |
Cxcl5 | NM_009141 | Forward 5’-TCGTGTTTGTCACTCGAAGG-3’ Reverse 3’-GGGATTACTGAGTGGCATCC-5’ |
Igfbp5 | NM_010518 | Forward 5’-CCTTGAGTGTGCCTCTGTCC-3’ Reverse 3’-ACAAGTTTGGGGGAGGTAGG-5’ |
Nos2 | NM_010927 | Forward 5’-GTGGTGACAAGCACATTTGG-3’ Reverse 3’-AAGGCCAAACACAGCATACC-5’ |
Pfkfb3 | NM_001177752 | Forward 5’-CAGCTACCAGCCTCTTGACC-3’ Reverse 3’-TGTACTCATTCTCGCCATGC-5’ |
Tnfa | NM_013693 | Forward 5’-AAAGGGGATTATGGCTCAGG-3’ Reverse 3’-CTCCCTTTGCAGAACTCAGG-5’ |
Tph1 | NM_009414 | Forward 5’-TTCACCATGATTGAAGACAAC-3’ Reverse 3’-TCCGACTTCATTCTCCAAGG-5’ |
aAll primers were designed using the Primer3 Input v.0.4.0. (http://bioinfo.ut.ee/primer3-0.4.0/).
GeneBank accession numbers are listed beside the primer name. Primer sequences are presented as 5’ to 3’ (forward) and 3’ to 5’ (reverse). Actb—β-actin; B2m—β-2-microglobulin; Hsp90ab1—Heat shock protein 90kDa Alpha Family Class B Member 1; Cck—cholecystokinin; Cxcl5 -chemokine (C-X-C motif) ligand 5; Igfbp5—insulin-like growth factor binding protein 5; Nos2—nitric oxide synthase 2; Pfkfb3—6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3; Tnfa—tumor necrosis factor α; Tph1—tryptophan hydroxylase 1.