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. 2016 Sep 7;11(9):e0162363. doi: 10.1371/journal.pone.0162363

Fig 2. Y. lipolytica CLIB89 YALI1 annotation pipeline.

Fig 2

YALI1 Annotations were derived from a combination of three automated annotation pipelines and a set of manual Blast searches. The three pipelines consist of mapping existing Yl annotations from CLIB122 YALI0 (NCBI RefSeq) to the CLIB89 YALI1 sequence; synteny and homology predictions (YGAP); and fungal HMM predictions (Snowy Owl). Loci of identified features were merged, checked for consistency, selected for CDS based on size and RNA-Seq support, and vetted through NCBI's Sequin upload service to produce the final set of gene features. Contributions and agreements for CDS features from the three automated pipelines are shown in the Venn diagram to the lower right.