Table 1. Genotypic and phenotypic characterization of MRSA and MSSA clinical isolates causing bovine mastitis in India.
| S.no | Isolate Id | Amp | Clin | Ery | Gen | Cip | Tet | Rif | Tei | Cef | Oxa MIC (μg/ml) | Van MIC (μg/ml) | Lin MIC (μg/ml) | mecA | SCCmec type | MLST* | Spa type | Agr type | pvl |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1. | TG-1 | R | R | R | R | S | R | S | S | S | <0.5 | 0.5 | 4 | + | V | ST-1687 | t7684 | 1 | + |
| 2. | TG-23 | R | R | R | S | R | R | R | S | S | <0.5 | 1 | 2 | + | NT | ST-2459 | t267 | 1 | + |
| 3. | TG-28 | R | R | R | R | R | S | S | S | S | 1.25 | 2 | 2 | + | V | ST-72 | t7287 | 1 | + |
| 4. | TG-29 | R | R | R | S | R | S | R | S | S | <0.5 | 1 | 2 | + | V | ST-72 | t7287 | 3 | + |
| 5. | TG-36 | S | S | S | S | S | S | S | S | S | <0.5 | 1 | 2 | + | III | ST-2459 | t267 | 1 | + |
| 6. | TG-52 | R | S | R | S | S | R | S | S | S | <0.5 | 2 | 2 | + | IVc | ST-239 | t037 | 1 | + |
| 7. | TG-55 | S | S | S | S | R | S | S | S | S | <0.5 | 1 | 2 | + | III+ IVc | ST-88 | t2526 | NT | + |
| 8. | TG-66 | R | R | R | S | S | S | R | S | S | 1.25 | 0.5 | 2 | + | III + IVc | ST-1687 | t7696 | 1 | + |
| 9. | TG-67 | R | R | R | S | S | S | R | S | S | <0.5 | 1 | 2 | + | III + IVc | ST-1687 | t7696 | 1 | + |
| 10 | TG-71 | R | S | R | S | R | R | S | S | S | 0.63 | 1 | 2 | + | IVc | ST-239 | t037 | 1 | + |
| 11 | TG-72 | R | R | R | S | R | R | S | S | S | 0.63 | 1 | 2 | + | IVc | ST-239 | t037 | 1 | + |
| 12 | TN-8 | R | R | R | S | S | S | S | S | S | <0.5 | 1 | 2 | + | III | ST-1687 | t2445 | 1 | + |
| 13 | TN-9 | R | R | R | S | R | S | S | S | S | <0.5 | 0.5 | 2 | + | III | ST-1687 | t7696 | 1 | + |
| 14 | TN-13 | S | R | R | S | S | S | S | S | S | <0.5 | 1 | 2 | + | III | ST-63 | t7287 | 3 | + |
| 15 | TN-38 | S | R | S | S | S | S | S | S | S | <0.5 | 1 | 2 | + | III | ST-2459 | t521 | 1 | + |
| 16 | AP-9 | R | R | R | R | R | R | S | S | S | <0.5 | 2 | 2 | + | V | ST-72 | t3731 | 1 | + |
| 17 | AP-20 | R | R | R | R | R | S | S | S | S | <0.5 | 2 | 2 | + | V | ST-72 | t8137 | 1 | + |
| 18 | AP-44 | R | R | S | R | S | S | R | S | S | 0.5 | 2 | 2 | + | NT | ST-72 | t7286 | 1 | - |
| 19 | AP-49 | S | R | S | S | R | S | S | S | S | 0.5 | 1 | 2 | + | III | ST-72 | t7286 | 1 | - |
| 20 | TG-33 | S | S | S | S | S | S | S | S | S | <0.5 | 1 | 4 | - | - | ND | t267 | 1 | + |
| 21 | TG-50 | R | S | R | S | S | R | S | S | S | <0.5 | 2 | 2 | - | _ | ND | t037 | 1 | + |
| 22 | TG-64 | R | S | S | S | S | S | S | S | S | <0.5 | 1 | 2 | - | _ | ND | t2164 | 2 | + |
| 23 | TG-74 | R | S | R | S | S | R | S | S | S | 0.63 | 1 | 2 | - | _ | ND | t037 | 1 | + |
| 24 | TN-1 | S | R | R | S | S | S | R | S | S | <0.5 | 1 | 2 | - | _ | ND | t7684 | 1 | + |
| 25 | TN-2 | S | R | S | S | S | R | S | S | S | <0.5 | 0.5 | 2 | - | _ | ND | t7684 | NT | - |
| 26 | TN-3 | R | R | R | S | S | R | R | S | S | <0.5 | 2 | 2 | - | _ | ND | t2445 | 3 | + |
| 27 | TN-7 | S | S | R | S | S | S | S | S | S | <0.5 | 0.5 | 2 | - | _ | ND | t7684 | 3 | - |
| 28 | TN-10 | R | R | R | S | R | S | S | S | S | <0.5 | 0.5 | 2 | - | _ | ND | t7696 | 1 | + |
| 29 | TN-15 | S | R | S | S | S | S | S | S | S | <0.5 | 1 | 2 | - | _ | ND | t7287 | 3 | + |
| 30 | TN-16 | R | R | R | S | R | S | S | S | S | 0.5 | 2 | 2 | - | _ | ND | t2445 | 1 | + |
| 31 | TN-19 | S | R | S | S | S | R | S | S | S | <0.5 | 1 | 2 | - | _ | ND | t9602 | 1 | + |
| 32 | TN-20 | R | R | R | R | S | R | R | S | S | <0.5 | 1 | 2 | - | _ | ND | t521 | 1 | + |
| 33 | TN-21 | S | R | R | S | R | R | S | S | S | <0.5 | 0.5 | 2 | - | _ | ND | t2246 | 1 | + |
| 34 | TN-27 | R | R | R | S | R | S | S | S | S | <0.5 | 0.5 | 2 | - | _ | ND | t2246 | 3 | + |
| 35 | TN-39 | S | R | S | S | S | S | S | S | S | <0.5 | 1 | 2 | - | _ | ND | t605 | 1 | + |
| 36 | AP-8 | S | R | S | S | S | R | S | S | S | <0.5 | 2 | 2 | - | _ | ND | t7286 | 2 | - |
| 37 | AP-23 | S | R | S | S | S | R | S | S | S | <0.5 | 2 | 2 | - | _ | ND | t7286 | 2 | - |
| 38 | AP-43 | S | R | S | S | S | S | S | S | S | <0.5 | 1 | 2 | - | _ | ND | t7287 | 3 | + |
| 39 | AP-45 | S | R | R | S | S | R | S | S | S | <0.5 | 2 | 2 | - | _ | ND | t7286 | 2 | - |
TG, TN and AP refer to strains isolated from Telangana, Tamil Nadu and Andhra Pradesh. The disc diffusion assay was performed with Amp (ampicillin, 10μg), Clin (clindamycin, 2 μg), Ery (erythromycin, 15 μg), Gen (gentamycin,10 μg), Cip (ciprofloxacin, 5μg), Tet (tetracycline, 30 μg), Rif (rifampicin,5 μg), Cef (cefoxitin, 30 μg) and Tei (teicoplanin, 30 μg). S denotes Sensitive and R denotes resistant strains against disc diffusion assay. Micro-broth dilution assays were performed for three drugs: Oxacillin (Oxa), Vancomycin (Van) and Linezolid (Lin). NT denotes non-typeable strains. + indicates positive for the gene and–denotes negative for the gene. No SCCmec typing was performed for mecA negative strains.
* MLST was only performed for MRSA clones, ND- Not determined.