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. 2016 Sep 7;11(9):e0162351. doi: 10.1371/journal.pone.0162351

Fig 10. Ph stability and proteolytic resistance of anti-VP6 VHH.

Fig 10

The graphs show the percentage of remaining functional 2KD1/3B2 after incubation in SGF (pepsin 0.32% w/v; pH 1.2) or SIF (pancreatin 1%, w/v; pH 6.8) in different conditions, determined by RVA VHH Ab titer ELISA. The panel above shows pH stability and proteolytic for 2KD1 clone whereas the panel below depicts these parameters for 3B2. In all cases 400 μg of the VHH were incubated for one hour at 37°C. Experiments were conducted by triplicate. SGF: Simulated Gastric Fluid. SIF: Simulated Intestinal Fluid. ORS: Oral Rehydration Salts.