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. 2016 Mar 8;5(3):e292. doi: 10.1038/mtna.2016.1

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Reduction of ANGPTL2 mRNA expression in normal cornea after topical administration of ANGPTL2 Li-pshRNA. Reverse transcriptase PCR showed that ANGPTL2 mRNA expression was significantly reduced in the corneal epithelium (a) at 12 and 24 hours (P = 0.049 and <0.001, respectively; n = 7) and in stromal cells (b) at 12 and 24 hours (P = 0.0030 and 0.0021, respectively, n = 7). Expression returned to baseline levels by 72 hours. *P < 0.05, **P < 0.01. ANGPTL2, angiopoietin-like protein 2; Li-pshRNA, proline-modified short hairpin RNA wrapped with liposome.