Figure 6.

Effect of a resting time on electrotransfer efficacy and toxicity in presence of small or large plasmids. Adipose tissue-derived mesenchymal stem cells were electrotransferred (eight pulses, 1,500 V/cm, 100 µs, 1 Hz) with 50 µg of either pCAGMKOSiE (11.4 kbp, 6.7 pmol) (filed squares and solid lines), pCXLE-eGFP (10.9 kbp, 7 pmol) (crosses and dashed line), or pCMV-GFP (3.5 kbp, 22 pmol) (open circles and dotted lines). After electrotransfer, cells were allowed to recover for 45 minutes (b,e) or for different amounts of time (a,c,d) before being put back in culture. Survival and transfection efficiency were assessed by flow cytometry 24 hours after treatment (a,c,d) or at several different days (b,e). In panel e, the dotted line represents the background fluorescence intensity of the controls. Data are representative of 3–11 independent experiments. CMV, cytomegalovirus; GFP, green fluorescent protein.