Figure 3.

Cell-type specificity of chimera treatment. (a) A549 and MCF7 cells were treated with 300 nM of GL21.T-miR212 for 48 hours. GL21.Tscr-miR212 and GL21.T aptamer were used as negative controls, whereas transfection with 100 nM of pre-miR-212 was used as positive control. Control scrambled was used to assure transfection efficiency. Cell lysates were immunoblotted with anti-PED and anti-β actin antibodies for PED protein levels while (b) PED expression levels were analyzed by qRT-PCR. (c) The same samples were subjected to qRT-PCR for miR-212 expression levels analysis. Bands' intensity has been calculated as in Figure 2. In b and c each bar shows the mean ± SD values from three wells. Statistics were calculated using Student's t-test, ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05.