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. 2016 Mar 8;5(3):e289. doi: 10.1038/mtna.2016.5

Figure 6.

Figure 6

Caspases activation induced by GL21.T-miR212. (a) A549 cells were transfected with 100 nM of pre-miR-212 or alternatively treated with 300 nM of GL21.T-miR212 for 48 hours. Scrambled miR and scrambled chimera were used as negative controls. Cells were, then, treated for 3 hours with TNF-related apoptosis-inducing ligand (TRAIL) 50 ng/ml, and cell lysates were immunoblotted with anti-caspase-8 antibody (upper panel). Band intensity is represented in the diagram of the lower panel as a ratio of cleaved over total caspase-8, both quantization normalized over β-actin. (b) A549 and MCF7 cells were transfected with pre-miR-212 or treated with the unconjugated aptamer, the scrambled chimera and GL21.T-miR212 for 48 hours and then incubated with 50 ng/ml of TRAIL for 6 hours. The activation of caspase 3/7 was measured by Caspase-Glo® 3/7 Assay. Each bar shows the mean ± SD values from three wells. Statistics were calculated using Student's t-test, ****p < 0.0001; ***p < 0.001; **p < 0.01.