Table 1. ANODEV table summary of GLM_MV models.

GLM_MV models were fitted to the response of experimental freshwater benthic communities exposed to selected PPCP low-dose mixtures and sequential analysis of deviance (ANODEV) was performed. MV data used to build each GLM_MV include the six community-level metabolic end points measured: F₀, the dark-adapted basal fluorescence; F, the light-adapted steady state fluorescence; Y_max, the maximum photosynthetic efficiency of photosystem II (PSII); Y_eff, the effective quantum yield of PSII; β-Glu, β-glucosidase; and Phos, alkaline phosphatase. The experiment included two factors: treatment (four levels) and time of exposure (three levels). Treatment levels were as follows: control (n = 5); Mix 16 (n = 3), PPCP mixture 16; Mix 16-4 (n = 3), mixture 16 without the four most important PPCPs from GSA results; and Mix 16/10 (n = 3), mixture 16 diluted 10 times. Time levels were as follows: 24, 36, and 120 hours of exposure for each treatment (n = 4). Therefore, the total number of samples was n = 42. The null hypothesis (H₀) for the Wald test is that the reduction in model residual deviance is 0. Res. df, residual degrees of freedom; df diff., degrees of freedom difference added by the sequential inclusion of each term of the model.

GLMMV model	Res. df	df diff.	Wald	P (>Wald)
Model 1 (full model): treatment × time
Intercept	41
Treatment	38	3	6.59	0.45
Time	36	2	13.05	0.86
Treatment × time	30	6	17.16	0.01**
Model 2 (control only): time
Intercept	14
Time	12	2	10.03	0.01**
Model 3 (Mix 16 only): time
Intercept	8
Time	6	2	15.75	0.54
Model 4 (Mix 16-4 only): time
Intercept	8
Time	6	2	162.2	0.04*
Model 5 (Mix 16/10 only): time
Intercept	8
Time	6	2	18.18	0.61

Significance values: *P < 0.05 and **P < 0.01.