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. 2015 Sep 3;1(1):vev008. doi: 10.1093/ve/vev008

Figure 2.

Figure 2.

Serial transfers in pkr -/- MEFs select for a small-plaque phenotype in PKR-positive cells. (A) Plaque assays of the T0 virus and line L2 (transfer 40) in BHK-21 cells and in pkr +/+ MEFs. (B) Fixation of the small-plaque phenotype (assayed in BHK-21 cells) during evolution. Approximately 100 plaques from a single plaque assay were counted to determine the small/total plaque ratio. Small plaques were not observed before passage 22 and reached quasi-fixation in all lines except L1 by transfer 35.