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. 2015 Sep 3;1(1):vev008. doi: 10.1093/ve/vev008

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© The Author 2015. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Individual fitness effects of parallel substitutions. (A) Competition assays of the T0 and single mutants against a common, phenotypically marked competitor in pkr -/- MEFs. Each line represents the change in the ratio of the assayed virus to the common competitor after a single 24 h transfer. Three replicates were performed for each competition. (B) Competition assays in pkr +/+ MEFs. (C) Correlation between fitness in pkr −/− and pkr +/+ cells. Error bars represent the standard error of the mean