Figure 3.

Rep gene phylogeny of smacoviruses, including genomes associated with stools from human and other animals. (A) The Bayesian phylogeny was generated using MrBayes, where 1,000,000 generations were sampled every 50 steps. Eleven clades were labeled as genogroups using 60 percent identity cutoff that was calculated by pairwise identities analysis. The scale bar indicates genetic distance. Branches were colored according to the animal from which the viral sequences were reported, and sequences from this study are annotated with an asterisk. (B) Frequency histogram of the pairwise identities between smacovirus Rep protein sequences. To avoid over-representation of human-associated smacoviruses, all animal-associated sequences and 3 selected human-associated sequences from Fig. 1 were used. (C) RCR motifs of smacoviruses comparing the 11 genogroups.