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. Author manuscript; available in PMC: 2016 Sep 7.
Published in final edited form as: Methods Enzymol. 2012;505:401–419. doi: 10.1016/B978-0-12-388448-0.00029-2

Figure 21.1.

Figure 21.1

A schematic description of metabolic labeling of cell surface glycans for fluorescence imaging. Red: an unnatural monosaccharide building block functionalized with a bioorthogonal chemical tag X; Green: a biophysical probe functionalized with a complementary chemical group Y. The covalent reaction between X and Y enables visualization or enrichment of the target glycoproteins for molecular identification.