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. 2016 Apr 19;5(4):e311. doi: 10.1038/mtna.2016.25

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Screen for effective H5N1 hemagglutinin-targeting artificial microRNAs. HEK 293T cells were transfected with influenza-specific amiRNA constructs (1 μg) and then infected with one clade of H5N1 at the dose of 0.0005 MOI 24 hours later (clade 2.3.2, clade 2.3.4, clade 7, or clade 9). Neg-miRNA plasmid transfectants were used as the positive controls. Influenza matrix gene expression was measured by real-time PCR 24 hours post-infection in cell lysate and normalized to β-actin. Matrix expression level in control groups was designated to be 100%, and the relative Matrix gene level in treated groups was normalized to be a percentage of the control value. Data were shown as mean relative expression to control ± SD from three independent experiments. ANOVA was applied to compare difference among groups. P < 0.01 in all IV-specific amiRNA transfectants when compared to positive controls. ANOVA, analysis of variance.