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. 2016 Apr 19;5(4):e311. doi: 10.1038/mtna.2016.25

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Suppression of H5N1 in vitro replication in the presence of rAd(HA-1405). (a) Expression of HA in adenovirus-treated Vero cells was determined by western blot 24 hours post H5N1 challenge in comparison to β-actin. Cells pretreated with rAd-empty served as controls. (b–e) Plaque reduction assay was conducted in MDCK cells. Cells were preincubated with 10-fold serially diluted rAd(HA-1405) (ranging from 10¹⁰ to 10⁸ vp) or rAd-empty (10¹⁰ vp). Twenty-four hours after treatment, 40–60 PFU of clade (b) 2.3.2, (c) clade 2.3.4, (d) clade 7, or (e) clade 9 H5N1 viruses were added to each well. The yields of viral progeny were determined by plaque assay. Data were shown as mean plaque counts ± SD from three independent experiments. ANOVA followed by Dunnett's test was used to compare the differences among different doses of rAs(HA-1405) groups and control group (PC). ** indicates P value was less than 0.01 in comparison to PC.