Figure 1.

LK-L1C/K6W/L8C increases microRNA-29b (miR29b) expression and enhances p53 activity in miR29-inducible HeLa cells. (a) Relative expression of mature miR29b was examined in the six candidate peptide-treated miR29-inducible HeLa cells by the Taqman miRNA assay. At 24 hours after treatment, mature miR29b expression was estimated. Mock indicates H₂O-treated cells as a control. Relative miR29b expression was normalized to the expression levels of U6 snRNA used as endogenous control. To estimate independent effects of miR29, the relative expression of miR29b in miR29-inducible HeLa cells treated with dox was first normalized to that of cells treated without dox in each peptide sample. Values for the relative miR29b expression of peptide-treated cells were further normalized to that of mock control (n = 3, mean ± SD). (b) Luciferase activity for p53 expression was analyzed by the pG13-luciferase assay. miR29-inducible HeLa cells were treated with the six candidate peptides. Luciferase activity was measured after 24 hours incubation and normalized to mock control, as demonstrated in Figure 1a. Data represent mean of the two independent experiments. (c) Summary of screening analysis for candidate peptides that enhance miR29b-induced p53 activity.