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. 2015 Dec 1;4(12):e265. doi: 10.1038/mtna.2015.40

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Copyright © 2015 Official journal of the American Society of Gene & Cell Therapy

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CD40, but not CD40L, is enriched in glomeruli and its glomerular expression is increased in DOX nephropathy. Glomerular and nonglomerular fractions were separated from collagenase-digested kidneys by cardiac injection of magnetic beads prior to organ harvest followed by positive selection of glomeruli using a magnet and negative selection of nonglomeruli collected as the flow-thru. (a) The glomerular-specific marker nephrin measured by quantitative real-time polymerase chain reaction (qRT-PCR) in whole kidney homogenates and the kidney fractions. Kidney fractions were prepared from healthy or DOX nephropathy mice and (b) CD40 and CD40L or (c) CD3d and CD68 qRT-PCR were performed in whole kidney homogenates and the kidney fractions. Data are normalized to whole kidney (−) DOX treatment = 1.0. Mean ± SEM, n = 4/group.