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Interstitial CD40 expression was primed for activation in kidneys with ureter obstruction and could be markedly reduced with CD40 antisense oligonucleotide (ASO) treatment. Kidney CD40 in situ hybridization performed in the healthy and obstructed kidneys in UUO mice receiving phosphate buffered saline (PBS) + CD40 mAb or 75 mg/kg CD40 ASO + CD40 mAb. Scale bar = 50 μm.
