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. 2015 Dec 8;4(12):e269. doi: 10.1038/mtna.2015.46

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Copyright © 2015 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Illustration of nucleic acid mimics (NAMs) hybridization to H. pylori and the different components of the implemented model. (a) Schematic representation of NAMs in gastric mucus, on their way to target H. pylori. (b) Monomers of the NAMs used in fluorescence in situ hybridization (FISH), compared to RNA (adapted from³²). (c) Illustration of the standard FISH procedure. PAR and ETH being paraformaldehyde and ethanol, respectively (adapted from ref. ³⁰). (d) Procedure followed for the collection of native mucus from the stomach of pigs, obtained from the slaughterhouse. ETH, ethanol; PAR, paraformaldehyde.