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. 2015 Dec 15;4(12):e270. doi: 10.1038/mtna.2015.47

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Copyright © 2015 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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miR-34a inhibits HR and RAD51 foci formation. (a) Homologous recombination assay in HeLa-DR-13–9 cells overexpressing miR-34a. HeLa-DR-13–9 cells were transfected twice on different days with scrambled sequences and miR-34a mimics. On day 6 after transfection, cells were collected and analyzed by flow cytometry. (b) Inhibition of RAD51 foci in A540 cells overexpressing miR-34a 8 hours after irradiation of 4 Gy are shown. (c) Reintroduction of the RAD51 ORF rescued the radiosensitization caused by miR-34a overexpression as measured by clonogenic survival assays. Cells were transiently transfected with miR-34a mimic or scrambled (±ORF). Cell viability was assessed using the clonogenic survival assay in the presence of radiation (2, 4, or 6 Gy). *P < 0.05 in a Student's t-test.