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. Author manuscript; available in PMC: 2016 Oct 4.
Published in final edited form as: Nat Neurosci. 2016 Apr 4;19(5):742–748. doi: 10.1038/nn.4285

Figure 4. SNR GABA neurons project to lateral SC.

Figure 4

(a) Lenti-FuGB2-Cre was injected into lateral SC yielding retrogradely-transported Cre. AAV-FLEX-GFP was injected in SNR, resulting in selective expression of GFP in nigrotectal neurons. Bottom inset shows nigrotectal axons in the lateral SC. (b) No overlap is observed between TH-expressing and GFP-expressing neurons. (c) GFP colocalizes with Vgat-expressing neurons. Experiment was conducted in a single mouse. (d-f) Nigrotectal projections were targeted with ChR2. AAV-Flex-ChR2-eYFP was injected into the SNR of Vgat-Cre mice. Optic fibers targeted the nigrotectal axons within the lateral SC. (d) Example of Vgat-ChR2-eYFP expression. (e) eYFP overlap with Vgat. (f) eYFP does not overlap with TH. Experiment was successfully repeated on 7 mice.