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. 2016 Aug 23;5:e17634. doi: 10.7554/eLife.17634

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© 2016, Roberts et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 4. — (A) nEBs consisting of 50% Ptch1^LacZ/LacZ;Ptch2^-/-;Shh^-/- cells (green) and 50% Shh^-/- cells or 50% Shh^-/-; 7dhcr ^-/- cells (magenta) were labeled with cell tracking vital dyes. (B) LacZ quantification in nEBs described in (A). 7dhcr ablation enhances non-cell autonomous inhibition of the Hh response in Ptch1^LacZ/LacZ;Ptch2^-/-;Shh^-/- cells. (C) The Hh response in Ptch1^LacZ/LacZ;Ptch2^-/-;Shh^-/- cells is inhibited by 7-Dehydrocholesterol (7DHC) as compared to cholesterol. The ability of 7DHC to inhibit the Hh response in Ptch1^LacZ/LacZ;Ptch2^-/-;Shh^-/- cells is exacerbated by the inclusion of Shh^-/- cells, and to a lesser extent by Shh^-/-;7dhcr^-/- cells. n > 8, *p<0.05, ***p<0.001, n.s., not significant (t-test).

DOI: http://dx.doi.org/10.7554/eLife.17634.007