Figure 7. 1,6-Hexanediol disrupts many cellular structures in yeast and alters cellular morphology in HeLa cells.
(A) The localization of various GFP-tagged proteins is shown either without treatment (No Drug); in the presence of 10% 1,6-Hexanediol and 10 μg/mL Digitonin for 2 min or 30 min; or just 10 μg/mL Digitonin for 2 min or 30 min. For Nsp1-GFP and Atp1-GFP, white lines depict cell boundaries. N. Pore = nuclear pore. ER = Endoplasmic Reticulum. Mito. = Mitochondria. Get1, Guided Entry of Tail-anchored proteins (YGL020C). Atp1, ATP synthase (YBL099W). Scale bar is 2 μm. (B) Time-lapse microscopy of HeLa cells treated with 3.5% 1,6-Hexanediol reveals alterations in cellular morphology. HeLa cells were exposed to 3.5% 1,6-Hexanediol (time zero) and imaged for 5 min using a 20X objective. Scale bars are 20 μm.