Fig E5.
RSV-induced IL-33 and ILC2s. WT or IL-33 KO mice were infected with 3 × 106 PFU of RSV strain 01/2-20, and lungs were harvested for ELISA or flow cytometry. A, ELISA for IL-33 in the whole-lung homogenate. B, Total number of live cells in the lungs at day 4 after infection. C, Total numbers of IL-13+ ILC2s as measured by using flow cytometry at day 4 after infection. D, ELISA for IL-13 in the whole-lung homogenate (right and left lung) at day 4 after infection. Data are plotted as means ± SEMs. For Fig E5, A, n = 5-10 mice per group combined from 2 independent experiments. For Fig E5, B and C, n = 13-17 mice per group combined from 3 independent experiments. For Fig E5, D, n = 4-5 mice per group from 1 independent experiment. *P < .05, **P < .01, and ***P < .001, 1-way (Fig E5, B and C) or 2-way (Fig E5, A) ANOVA. n.s., Not significant.