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. Author manuscript; available in PMC: 2017 Sep 12.
Published in final edited form as: Angew Chem Int Ed Engl. 2016 Aug 11;55(38):11577–11581. doi: 10.1002/anie.201606242

Table 1.

Kinetic parameters of the Pyrococcus furiosus and Archaeoglobus fulgidus TrpB wild-type enzymes and 0B2 variants.[a]

Entry Enzyme kcat
(s−1)		 KM
(μM indole)		 kcat/KM
(μM−1 s−1 indole)
1 PfTrpBWT 0.31 77 4.0
2[b] PfTrpB0B2 2.9 9 300
3 AfTrpBWT 0.074 12 6.0
4[c] AfTrpB0B2 0.51 4.8 110
[a]

Assays conducted in potassium phosphate buffer (pH 8) at 75 °C for Pf and 60 °C for AfTrpB. See SI section 5.4 for experimental details. Standard errors are in Table S1.

[b]

Contains the mutations P12L, E17G, I68V, F274S, T292S, and T321A.

[c]

Contains the mutations P25L, P30G, I80V, L285S, T303S, and T321A.