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. Author manuscript; available in PMC: 2017 Sep 12.
Published in final edited form as: Angew Chem Int Ed Engl. 2016 Aug 11;55(38):11577–11581. doi: 10.1002/anie.201606242

Table 2.

Kinetic parameters of Thermotoga maritima TrpB variants and TrpS.[a]

Entry Enzyme kcat
(s−1)		 KM
(μM indole)		 kcat/KM
(μM−1 s−1 indole)
1 TmTrpBWT 1.28 33 39
2 TmTrpB0B2 [b] 0.11 72 2.0
3 TmTrpBtriple [c] 9.8 26 380
4 TmTrpBT292S 5.8 25 230
5 TmTrpS 2.2 44 50
[a]

Assays conducted in potassium phosphate buffer (pH 8) at 75 °C. See SI section 5.4 for experimental details. Standard errors are in Table S1.

[b]

Contains the mutations P14L, P19G, I69V, L274S and T292S.

[c]

Contains the mutations P19G, I69V, and T292S.