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. Author manuscript; available in PMC: 2017 Sep 6.
Published in final edited form as: Biochemistry. 2016 Aug 23;55(35):4939–4948. doi: 10.1021/acs.biochem.6b00599

Figure 4.

Figure 4

Effect of temperature on ApoC-III folding during nanodisc self-assembly. (A) Mean residue ellipticity (MRE) of WT ApoC-III in the absence (open squares) and presence of DMPC (solid squares) at 45 °C (left) and 25 °C (right). The position of 222 nm is denoted by the arrow. Units for MRE (deg cm2 dmol−1) were omitted for clarity. (B, upper panel) MRE as a function of temperature for WT ApoC-III and Trp mutants (10 μM) in the presence of DMPC MLVs (500 μM). WT (black), W42 (red), W54 (green), or W65 (blue) ApoC-III was incubated at 45 °C (15 min) followed by the addition DMPC MLVs. Temperature was scanned (20 °C/h) from 45 to 25 °C as indicated by the arrow. (B, lower panel) CD temperature scans of WT (black), W42 (red), W54 (green), or W65 (blue) ApoC-III in buffer alone (10 mM Tris, 100 mM NaCl, 1 mM EDTA, pH 7.4).