Figure 2. Interaction between eEF-2K_CBD with CaM in the presence of Ca²⁺.

(A) ITC trace representative of three repeats is shown. For the trace shown, K_D=137.2±18.8 nM, ΔH=−16.6 KCal•mol⁻¹, ΔS=−22.5 Cal•mol⁻¹•K⁻¹. (B) Backbone amide chemical shift perturbations induced on Ca²⁺-CaM upon formation of the 1:1 Ca²⁺-CaM•eEF-2K_CBD complex. The locations of the eight helices (A, B, C, D on the N-lobe and E, F, G, H on the C-lobe) are shown. The blue bar represents residues that are unassigned in the Ca²⁺-CaM•eEF-2K_CBD complex.