| The following antibodies were used for immunostaining: | |||
|---|---|---|---|
| Antibody name | Company | Cat# | Dilution |
| Angiopoietin-1, goat | Abcam | ab133425 | 1:200 |
| Angiopoietin-2, goat | R&D | AF623 | 1:100 |
| Angiopoietin-2, rabbit | Abcam | ab8452 | 1:200 |
| Angiopoietin-2, rabbit | Novus | NBP2-1538 | 1:100 |
| Beta-Catenin, mouse | BD Pharmingen | 51-9001921 | 1:100 |
| CCM3, mouse | Santa Cruz | SC365587 | 1:1000 |
| CCM3, rabbit | Min lab | 1:1000 | |
| CD31, rat | BD Pharmingen | 553370 | 1:100 |
| CD31, rabbit | Abcam | ab28364 | 1:100 |
| Claudin-5, rabbit | Invitrogen | 34-1600 | 1:100 |
| Collagen IV, rabbit | AbD Serotec | 2150-1470 | 1:300 |
| Collagen IV, rabbit | Abcam | ab6586 | 1:500 |
| Connexin-43, mouse | BD Pharmingen | 51-9001918 | 1:100 |
| NG2, rabbit | Millipore | AB5320 | 1:100 |
| Phospho-TIE2 (Tyr992), rabbit | Cell Signaling | 4226 | 1:1000 |
| Phospho-Tie2 (Y992), rabbit | R&D | AF2720 | 1:100 |
| TIE2, rabbit | Cell Signaling | 4224 | 1:1000 |
| VAMP3, goat | Santa Cruz | Sc-18208 | 1:100 |
| VE-cadherin, rat | BD Pharmingen | 555289 | 1:100 |
| VE-cadherin, goat | Santa Cruz | sc-6458 | 1:100 |
| ZO-1, rabbit | Invitrogen | 61-7300 | 1:100 |
| Alexa Flour 488 Phalloidin | Invitrogen | A12379 | 1:1000 |
| Alexa Flour 594 Isolectin GS-IB4 conjugate |
Invitrogen | I21413 | 1:50 |
| Alexa Flour 488 Donkey Anti-Rat IgG | Invitrogen | A21208 | 1:200 |
| Alexa Flour 488 Donkey Anti-Goat IgG | Invitrogen | A11055 | 1:200 |
| Alexa Flour 488 Donkey Anti-Rabbit IgG | Invitrogen | A21206 | 1:200 |
| Alexa Flour 488 Donkey Anti-Mouse IgG | Invitrogen | A21202 | 1:200 |
| Alexa Flour 594 Donkey Anti-Rat IgG | Invitrogen | A21209 | 1:200 |
| Alexa Flour 594 Donkey Anti-Goat IgG | Invitrogen | A11058 | 1:200 |
| Alexa Flour 594 Donkey Anti-Rabbit IgG | Invitrogen | A21207 | 1:200 |
| Alexa Flour 594 Donkey Anti-Mouse IgG | Invitrogen | A21203 | 1:200 |
The following antibodies were used for Western blot:
Rabbit polyclonal antibody against CCM3 was generated (Invitrogen) against full-length recombinant human CCM3 protein expressed and purified from Escherichia coli. Beta-Actin is from Sigma (mouse, A1978); GAPDH (rabbit, 2118), p-Smad2 (rabbit, 3180), p-MLC (rabbit, 3674), VEGFR2 (rabbit, 2479), p-TIE2 (rabbit, 4221), TIE2 (rabbit, 4224), p-FAK (rabbit, 3281) are from Cell Signaling Technology; Beta-Catenin (mouse, sc-7963), Cdc42 (mouse, sc-8401), Rac1 (sc-217), RhoA (mouse, sc-418), STK25 (goat, sc-6865) and VAMP-3 (goat, sc-18208) are from Santa Cruz Biotechnology. ANGPT2 (rabbit, Ab8452) is from Abcam and ANGPT2 (AF7186) from R&D; Integrin beta-1 (mouse, 610467) and FAK (mouse, 610087) are from BD. All first antibodies were used at 1:1000 dilution.
The uncropped gel images for Western blotting are presented at Supplementary Data Set 1.