q-RTPCR of claudin-4, claudin-8, and occludin mRNA expression relative to β-actin. Values are mean ± SEM of 4-7 animals per group expressed relative to sham. **, p<0.01 burn day one claudin-4 compared to sham saline by Student's t-test. *, p<0.05, **, p<0.01, ***, p<0.001 burn day one claudin-8 and occludin compared to sham saline by ANOVA and Tukey post hoc test.