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. Author manuscript; available in PMC: 2016 Sep 7.
Published in final edited form as: Cell Rep. 2016 Aug 25;16(10):2723–2735. doi: 10.1016/j.celrep.2016.08.002

Figure 1. Innervation of the developing mouse hindlimb by TrkA sensory nerves.

Figure 1

TrkA-LacZ embryos were subjected to X-Gal staining, then imaged intact at A) E14.5 B) E16.5 C) E18.5 and D) P0. Hindlimbs were then removed and imaged separately to illustrate the medial aspect of the femur at E) E14.5 F) E16.5 G) E18.5 and H) P0. Femurs from Thy1-YFP embryos were carefully stripped of soft tissue, optically cleared, then imaged by confocal microscopy at I) E14.5 J) E16.5 K) E18.5 and L) P0.5, with high powered insets (M–P) that show the progressive arborization of nerves on the surface of the bone. Arrows indicate TrkA-LacZ+ nerve axon at the perichondrial region. Scale bars are 100 microns.