Skip to main content
. Author manuscript; available in PMC: 2017 Sep 6.
Published in final edited form as: Structure. 2016 Aug 18;24(9):1562–1572. doi: 10.1016/j.str.2016.07.001

Fig. 5. Recombinant influenza B viruses with mutations in the CTD RNA-binding domain of NS1 all inhibit IRF3 activation.

Fig. 5

A549 cells were infected with 5 pfu / cell of wt influenza B virus, a mutant virus encoding the N-terminal 104 amino acids of the NS1 protein, or a mutant virus expressing a NS1 protein with one of the indicated mutations in the NS1 protein. At the indicated times after infection, cell extracts were immunoblotted with antibodies against activated (phosphorylated) IRF3 (Cell Signaling), total IRF3, actin, viral NS1, or M1 protein.