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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: J Allergy Clin Immunol. 2016 Apr 6;138(3):769–779. doi: 10.1016/j.jaci.2016.01.049

Figure 5. NSG-SGM3 BLT PEC cells bind human IgE, and degranulate in an IgE-dependent and independent manner.

Figure 5

Histograms of sensitized NSG-SGM3 BLT PEC cultures (>90% mast cells) (A) incubated with NP-BSA or Iono (B). (C) Calcium flux of panel A cells. (D) MFI replicates of panel C. Pre: baseline values, NP: NP-BSA, Iono: ionomycin, representative of two independent experiments. (E) NSG-SGM3 PEC mast cell day 34 cultures (20,000 mast cells/well) treated with ionomycin (2 uM) or varying concentrations of Substance-P, C3a, C5a. (F) Percent CD107a cells.

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