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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Methods. 2016 Mar 24;107:73–78. doi: 10.1016/j.ymeth.2016.03.016

Table 1.

Neutral loss occurs when a labile nucleobase fragments instead of the phosphodiester backbone. The neutral losses in this table are those that have been found to dominate MS/MS spectra when present in oligoribonucleotides, often making sequence determination difficult. Key: m7G – 7-methylguanosine; k2C – lysidine; Q – queuosine; oQ – epoxyqueuosine; t6A – N6-threonylcarbamoyladenosine

Ion Neutral Loss (u)
PO3H2 80.974
[m7G] 165.065
[k2C] 144.090
[Q] 115.064
[oQ] 131.058
[t6A] 147. 053/162.064