Table 1.
Neutral loss occurs when a labile nucleobase fragments instead of the phosphodiester backbone. The neutral losses in this table are those that have been found to dominate MS/MS spectra when present in oligoribonucleotides, often making sequence determination difficult. Key: m7G – 7-methylguanosine; k2C – lysidine; Q – queuosine; oQ – epoxyqueuosine; t6A – N6-threonylcarbamoyladenosine
| Ion | Neutral Loss (u) |
|---|---|
| PO3H2 | 80.974 |
| [m7G] | 165.065 |
| [k2C] | 144.090 |
| [Q] | 115.064 |
| [oQ] | 131.058 |
| [t6A] | 147. 053/162.064 |